His write-up as: Leroy et al.: Estimating time-to-onset of adverse drug reactions from spontaneous reporting databases. BMC Health-related Research Methodology 2014 14:17.Submit your next manuscript to BioMed Central and take complete advantage of:?Practical on-line submission ?Thorough peer overview ?No space constraints or color figure charges ?Immediate publication on acceptance ?Inclusion in PubMed, CAS, Scopus and Google Scholar ?Research which can be freely accessible for redistributionSubmit your manuscript at biomedcentral/submit
NIH Public AccessAuthor ManuscriptNanoscale. Author manuscript; obtainable in PMC 2014 April 07.Published in final edited type as: Nanoscale. 2013 April 7; five(7): 2664?668. doi:ten.1039/c3nr00015j.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author Manuscript`Marker-of-self’ functionalization of nanoscale particles by way of a top-down cellular membrane coating approachChe-Ming J. Hua,b, Ronnie H. Fanga,b, Brian T. Lukb,c, Kevin N.H. Chena, Cody Carpentera, Weiwei Gaoa,b, Kang Zhanga,d,e, and Liangfang Zhanga,bLiangfang Zhang: [email protected] NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. Tel: 1-858-246-bMooresCancer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. Tel: 1-858-246-cDepartmentof Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. Tel: 1-858-246-dDepartmentof Ophthalmology and Shiley Eye Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. Tel: 1-858-246-eStateKey Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510275, ChinaAbstractWe investigate the `marker-of-self’ functionalization of nanoparticles via coating of all-natural RBC membranes.92220-65-0 Formula The membrane translocation approach is shown to become extremely efficient and bestows nanoparticles with correctly oriented and functional immunomodulatory proteins such as CD47 at equivalent density to natural RBCs.Price of Fmoc-OSu Enabling active immune evasion via biomimetic surface functionalization presents an emerging stealth technique for developing long-circulating delivery cars.PMID:23398362 1, 2 The identification of CD47, a transmembrane protein that serves as a universal molecular `marker-of-self’, has led to its utilization inside the growing development of bio-inspired, immune-evasive devices. Capable of inhibiting phagocytosis and conferring antiinflammatory properties through interactions with signal regulatory protein alpha (SIRP) expressed by macrophages, CD47 and its analogs have been found to contribute to the in vivo survival of red blood cells (RBCs),3 cancer cells,four and viruses5. Application of CD47 to modulate the immune responses against synthetic devices was 1st demonstrated with macrophages treated by purified recombinant, soluble CD47, which showed reduced uptake of colloidal emulsions.6 Synthetic supplies covalently conjugated with recombinant CD47 additional sophisticated this biomimetic stealth approach, yielding polymeric microspheres7 and implant surfaces with reduced affinity to inflammatory cells.eight, 9 On nanoscale particles, nonetheless, interfacing with native biological elements by means of chemical conjugation of immunomodulatory proteins to particle surfaces is usually hard to manipulate. In particular, inconsistent protein surface density and randomized ligand orientations are notable troubles that could greatly undermine the performance from the re.
